In vivo and ex vivo longitudinal follow-up of resveratrol supplementation or restauration of a normal diet in female rat hearts submitted to high-fat-high-sucrose diet

Prediabetic women are at greater risk of cardiovascular diseases than men, investigating new sex dependent strategic therapies is then essential to limit complications in prediabetic women. We aimed evaluate the effects resveratrol supplementation (RSV) or restauration a standard diet on heart female rats submitted high-fat-high-sucrose (HFS). Wistar were divided 4 groups fed for 5 months with: normal HFS (CTRL/HFS), with RSV (1 mg/kg/day) during last 2 3 and (RSD). performed longitudinal vivo study cardiac function, morphology perfusion by MRI, underwent an IPGTT. Ex experiments isolated perfused hearts realized function [rate pressure product (RPP), end diastolic (EDP)] energy metabolism 31P magnetic resonance spectroscopy ischemia-reperfusion injury (IR). Tissues collected analyzes. Five diet-induced glucose intolerance (P < 0.05), increased heart/tibia length ratio (HTLR) 0.01 vs. CTRL). also induced altered myocardial tolerance IR, characterized impaired RPP EDP 0.001) associated lower ATP PCr levels reperfusion 0.001, P 0.05 had no effect CTRL) but normalized HTLR CTRL level. Interestingly, diminished HFS). RSD without improved IR HFS), 0.01, 0.001 The two approaches have distinct rats. Further studies go explore mechanisms involved.